Tuesday, June 22, 2010
Second Day First Lab
Today we had a three hour lab so that we could learn how to work different types of pipettes and work with bacteria cultures.
I really enjoyed getting to take a lot of time In the lab because in my AP Bio class we only had 45 minutes to complete a lab that might take an hour to an hour and a half.
We learned how to calibrate a small-volume micropipettor along with a large-volume micropipettor. I had a little trouble at first with getting the right measurements but once I got that part of the lab down I was on my way. I messed up the whole lab about two times and like they say, third time's a charm. We had to measure certain amounts of certain liquids into specific tubes and I kept putting the wrong amount of liquid in the tube, or forgetting if I had put the liquid in. Once I got the right liquids in the right place I used a microfuge for the fist time. I learned that you have to balance it out by placing the tubes equal distances apart.
I was really glad that I was able to do this lab because if I needed to do this for a lab that affected my grade I would have really had a hard time.
The professor realizes that some of us have not used any of the equipment so I appreciated the opportunity.
There was also the other part of the lab where we had to transfer two different strands of E. coli - one ampacilin resistant and the other not. I placed both strands on a tray with ampacilin and one with agar. Agar is just food for the bacteria. I have to wait to see if I did it correctly tomorrow because it needs to be incubated overnight.
I can't wait for tomorrows lab!